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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been observed in lots of tumors, which leads to chromosome instability, and thus may contribute to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential part in advertising mobile-cycle development in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two extra splice variants were being documented but comprehensive nucleotide sequence information is not really nonetheless available.
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Blocking peptides are peptides that bind exclusively to your target antibody and block antibody binding. These peptide typically includes the epitope recognized by the antibody.
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This manual outlines further probable optimization of mobile and immunoassay parameters to make certain the best possible success are...
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This guidebook outlines further attainable optimization of mobile and immunoassay parameters to make sure the very best results are...
Antibodies certain to the blocking peptide no more bind to the epitope within the focus on protein. By evaluating the staining from the blocked antibody versus the antibody on your own, one can see which staining is particular.
Target Information Cyclin E belongs towards the very conserved cyclin household, whose customers are characterized by a remarkable periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation styles which add for the temporal coordination of each and every mitotic function. Cyclin E types a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S transition. This protein accumulates within the G1-S phase boundary and is degraded as cells development by S period.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both equally antibodies...
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